새로운 적외선 온열기의 열충격 단백질 유도

목적 : 임상적으로 많이 사용하고 있는 적외선 온열기의 사용후 열충격 단백질을 유도하는지 알아보고자 하였다.<BR> 대상과 방법 : 온열치료는 20분간 실시하였다. 치료전후에 상안검 및 각막 중심부 온도를 측정하고, 적외선체열진단기로 치료후 영상을 얻었다. 온열치료 6시간후에 nitrocellulose paper를 결막 표면에 압력을 주어 부착하여 표본을 얻어 면역염색을 시행하였다. 온열치료전후에 눈물 표본을 얻어 Heat shock protein (HSP) 27, 47, 70, 90에 대한 western blot을 시행하였다. <BR> 결과 : 면역화학염색 및 western blot검사에서 HSP 70과 HSP 27의 발현이 온열치료 후 유의하게 유도되었다. 그러나 면역화학염색검사에서 47과 90은 온열치료전후간에 차이를 보이지 않았다. western blot 검사상 HSP 47은 열충격에 의해 아주 미약한 정도로 증가하였고 HSP 90은 온열군과 대조군간에 유의한 차이가 없었다.<BR> 결론 : 적외선 온열기가 HSP 27, 70의 발현을 유의하게 유도하였으며, HSP 47도 미약하나마 발현함을 확인 할 수 있었다. 이는 아마도 염증으로 인한 세포손상을 줄이는 효과가 있을 것으로 기대된다.〈한안지 45(5):875-882. 2005〉

Purpose: To investigate if the use of the infrared warm compression device often used in clinical settings induced heat shock proteins.<BR> Methods: Subjects were heat-treated with an infrared warm compression device for 20 minutes. We examined the temperature of the upper eyelid and cornea before and after heat treatment and images were obtained by Digital Infrared Thermal Imaging System. After 6 hours of heat treatment, conjunctival epithelial cells were obtained by gently pressing nitrocellulose paper on the conjunctival surface for 3 to 5 seconds Immunocytochemical staining analysis was performed on the obtained samples. Tear samples were obtained prior to heat treatment and Western blot was performed to observe the expression patterns of heat shock proteins 27, 47, 70, and 90.<BR> Results: By Western blot and immunocytochemical analysis, heat shock proteins 70 and 27 were significantly increased in the heat-treated samples. However, no difference was observed for heat shock proteins 47 and 90 before and after heat treatment, according to the immunocytochemical analysis.<BR> On Western blot, heat shock protein 47 was slightly increased by heat treatment but heat shock protein 90 did not show a significant difference after heat treatment.<BR> Conclusions: It was observed that the infrared warm compression device significantly increased the induction of heat shock proteins 27 and 70, and that 47 was also slightly induced. This result suggests that the device developed herein could be used as a new therapeutic modality for the reduction of inflammatory cell injury through the induction of heat shock proteins. J Korean Ophthalmol Soc 46(5):875-882, 2005<BR>