산천어(Oncorhynchus masou masou) 정자의 냉동보존에 미치는 희석액과 동해방지제의 영향

Effects of Diluents and Cryoprotectants on Sperm Cryopreservation of Mason Salmon, Oncorhynchus masou masou

We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained $18{\pm}1mmol/L$ potassium, $144{\pm}4mmol/L$ sodium, $116{\pm}3mmol/L$ chloride, $83.2{\pm}3.1mg/L$ calcium, $14.8{\pm}0.7mg/L$ magnesium, $45{\pm}9mg/L$ glucose, and $1.0{\pm}0.0g/L$ total protein. The osmolality and pH of seminal plasma were $287{\pm}7\;and\;7.7{\pm}0.1mmol/kg$, respectively, and the spermatocrit was $28{\pm}2$. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300 mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.