장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴
Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes
- 한국미생물·생명공학회
- Microbiology and Biotechnology Letters
- Vol.38 No.1
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2010.0170 - 76 (7 pages)
- 0
The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.
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