Identification of Insecticidal Compounds from Actinomycetes Isolate No. 1166

In the course of screening for insecticidal metabolites from soil microorganisms, Actinomycetes isolate no.1166 was found to produce active metabolites against Musca domestca and Bombyx mori. Three active components from the metabolites were isolated by solvent extraction and chro- matographic techniques and examined their insecticidal activities on Bombyx mori (3rd larvae) by diet feeding bioassay methods. By UV and NMR data analyses, compound I and III were identified as bafilomycin A$_{2}$ and B$_{1}$, respectively and compound II was also estimated to belong to the bafilomycin family from its physico-chemical data and biological properties.