Digitalis lanata 현탁세포배양에서의 생물학적 변환을 이용한 Digoxin 생산

Digoxin Production by Using Biotransformation in Digitalis lanata Cell Suspension Cultures

For the production of digoxin by using biotransformation in suspension-cultured Digita- lis lanata cells, a two-stage culture process was optimized. Modified Murashige and Skoog medium was used for growth in the first stage and the cells were transferred to glucose solution for the production of digoxin from digitoxin via biotransformation in the second stage. When the cells were cultivated for 10 days in the growth period, 12$\beta$-hydroxylation capacity was the best. It was found that the optimum amount of digitoxin as substrate was 400 mg/l with initial cell density of 21%. Maximum productivity was achieved 5 days after transfer of cells to production medium. Sucrose and fructose provided similar digoxin yield as that in glucose, and 6% was proved to be the best glucose solution. Most of the components of modified MS medium except phosphate reduced the efficiency of digoxin formation. Besides, peptone and beef extracts inhibited 12$\beta$-hydroxylation, while promoting glucosylation. Finally, it was apparent that light enhanced the formation of digoxin significantly.