Burkholderia cepacia Strain G4 (pHG-2) Accumulates cis-3-Methyl-3,5-cyclohexadien-1,2-diol While Growing on Toluene

Burkholderia cepacia Strain G4 (pHG-2) Accumulates cis-3-Methyl-3,5-cyclohexadien-1,2-diol While Growing on Toluene

Burkholderia cepacia strain G4 (pHG-2) containing toluene 2-monooxygenase and toluene dioxygenase, was able to grow on toluene and accumulate cis-3-methyl-3,5-cyclohexadien-1,2-diol (cis-toluene dihydrodiol) in the liquid culture. The cis-toluene dihydrodiol produced was identical to the authentic compound, as judged through mass spectrometry and nuclear magnetic resonance analysis. Our results indicate that pHG-2 provides an economical means to produce chemically-important chiral synthons while growing on toluene.

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