<TEX>$^1H$</TEX> NMR Studies of the Interaction between Cytochrome c3 and ferredoxin I from D. Vularis Miyazaki F

Heme assignment of the 1HNMR spectrum of cytochrome c3 of D. vulgaris Miyazaki F was established [Reference: 12, 13]. The major reduction of the heme turned out to take place in the other of heme 4, 1, 2 and 3 (in the sequential numbering). The Hemes with the smallest and greatest solvent accessibility were reduced at the highest and lowest potentials in average, respectively. A cooperation interheme interaction was attributed to a pait of the closest hemes, namely, hemes 1 and 2. This assignment can provide the physicochemical bases for the elucidation of electron transfer of this protein.