Aspergillus niger KUF-04가 생산하는 Catalase

Catalase from Aspergillus niger KUF-04

Aspergillus niger KUF-04에서 얻은 catalase는 gel 여과를 포함하여 5단계를 걸쳐 정제하였으며, 9% 회수율로 64배 정제되었다. 본 효소는 406, 503, 625nm에서 흡광을 나타내었으며, 특히 406nm에서 뚜렷한 흡수대를 보여주었다. 이 효소 활성의 최적 pH와 온도는 각각 7.0과 6<TEX>$0^{circ}C$</TEX>이었다. 이 효소는 pH4.0과 8.0 사이에서 안정했으며 열에 대한 안정성은 2<TEX>$0^{circ}C$</TEX>에서 5<TEX>$0^{circ}C$</TEX>까지는 안정했으나, 8<TEX>$0^{circ}C$</TEX>에서 20분간 반응시켰을 때 효소의 활성이 전부 소실되었다. 이 효소의 활성은 주로 hydroxylamine, potassium cyanide, sodium azide에 의해 저해되었다.

Catalase from Aspergillus niger KUF-04 was purified by five steps including gel filtration. The overall purification gave 64-fold purified preparation, a yield of about nine percent. The enzyme showed its maximum absorption at 406 nm. The optimum pH and temperature for the enzyme activity were around pH 7.0 and 6<TEX>$0^{circ}C$</TEX>, respectively. The catalase was found to be stable in the range of pH 4.0 to pH 8.3 and temperature 2<TEX>$0^{circ}C$</TEX> to 6<TEX>$0^{circ}C$</TEX>. However, it lost nearly all of the activity by heating at 8<TEX>$0^{circ}C$</TEX> for 20 min. The activity was markedly inhibited by hydroxylamine, potassium cyanide and sodium azide.