Cytoprotective Constituents of Alpinia katsumadai Seeds AgainstGlutamate-Induced Oxidative Injury in HT22 Cells

Cytoprotective Constituents of Alpinia katsumadai Seeds AgainstGlutamate-Induced Oxidative Injury in HT22 Cells

oxidative injury contributes to neuronal degeneration in many central nervoussystem (CNS) diseases, such as epilepsy and ischemia. Bioassay-guided fractionation of the MeOH extract of theseeds of Alpinia katsumadai 1),pinocembrin (2), and (+)-catechin (3). Compounds 2 and 3 showed the potent neuroprotective effects onglutamate-induced neurotoxicity and reactive oxygen species (ROS) generation in the mouse hippocampal HT22cells. In addition, Compounds 2 and 3 showed significant DPPH free radical scavenging effect. These results suggestthat compounds 2 and 3KeywordsGlutamate-induced toxicity, Alpinia katsumadai, HT22, DPPH, Cytoprotective

oxidative injury contributes to neuronal degeneration in many central nervoussystem (CNS) diseases, such as epilepsy and ischemia. Bioassay-guided fractionation of the MeOH extract of theseeds of Alpinia katsumadai 1),pinocembrin (2), and (+)-catechin (3). Compounds 2 and 3 showed the potent neuroprotective effects onglutamate-induced neurotoxicity and reactive oxygen species (ROS) generation in the mouse hippocampal HT22cells. In addition, Compounds 2 and 3 showed significant DPPH free radical scavenging effect. These results suggestthat compounds 2 and 3KeywordsGlutamate-induced toxicity, Alpinia katsumadai, HT22, DPPH, Cytoprotective