Accelerating Effects of Quercetin on the TNF-α-Induced Apoptosis in MC3T3-E1 Osteoblastic Cells

Accelerating Effects of Quercetin on the TNF-α-Induced Apoptosis in MC3T3-E1 Osteoblastic Cells

Bioflavone quercetin is believed to play an important role preventing bone loss by afectingosteoclastogenesis and regulating many systemic and local factors including hormones and cytokines. This studyexamined how quercetin acts on tumor necrosis factor-alpha (TNF-α)-mediated apoptosis in MC3T3-E1osteoblastic cells. Apoptosis assays revealed the dose-dependent acceleration of quercetin on TNF-α-inducedapoptosis in MC3T3-E1 cells, which was demonstrated by the increased number of positively stained cells in thetrypan blue staining and TUNEL assay, and the migration of many cells to the sub-G0/G1 phase in flow cytometricanalysis. In particular, quercetin treatment alone increased the expresion of p53 and p21 proteins in the cells.Consequently, this study showed that quercetin accelerates the TNF-α-induced apoptosis in MC3T3-E1osteoblastic cells.

Bioflavone quercetin is believed to play an important role preventing bone loss by afectingosteoclastogenesis and regulating many systemic and local factors including hormones and cytokines. This studyexamined how quercetin acts on tumor necrosis factor-alpha (TNF-α)-mediated apoptosis in MC3T3-E1osteoblastic cells. Apoptosis assays revealed the dose-dependent acceleration of quercetin on TNF-α-inducedapoptosis in MC3T3-E1 cells, which was demonstrated by the increased number of positively stained cells in thetrypan blue staining and TUNEL assay, and the migration of many cells to the sub-G0/G1 phase in flow cytometricanalysis. In particular, quercetin treatment alone increased the expresion of p53 and p21 proteins in the cells.Consequently, this study showed that quercetin accelerates the TNF-α-induced apoptosis in MC3T3-E1osteoblastic cells.