Molecular Characterization and Physico-Chemical Analysis of a New Giant Embryo Mutant Allele (get) in Rice (Oryza sativa L.)

Molecular Characterization and Physico-Chemical Analysis of a New Giant Embryo Mutant Allele (get) in Rice (Oryza sativa L.)

The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a new giant embryo mutant, get, induced from somaclonal variation derived by anther culture in rice. Sequence analysis of Cytochrome P450 of the get mutant revealed that get is a new allele of the GE gene with a single point mutation with substitution of amino acid, W395 to L395. The weight of the get mutant embryo was 3.7 times higher than normal embryo. Tocopherol and mineral content were also higher than the previously reported giant embryo rice variety, Keunnun. These results indicated that this new giant embryo rice ( get) offers a promising source of genetic material in improving nutritional quality of rice especially tocopherol, essential minerals, and GABA.

The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a new giant embryo mutant, get, induced from somaclonal variation derived by anther culture in rice. Sequence analysis of Cytochrome P450 of the get mutant revealed that get is a new allele of the GE gene with a single point mutation with substitution of amino acid, W395 to L395. The weight of the get mutant embryo was 3.7 times higher than normal embryo. Tocopherol and mineral content were also higher than the previously reported giant embryo rice variety, Keunnun. These results indicated that this new giant embryo rice ( get) offers a promising source of genetic material in improving nutritional quality of rice especially tocopherol, essential minerals, and GABA.