지리산 자생식물 5종의 화장품 원료개발을 위한 성분 및 DPPH, ABTS 활성분석-Ⅲ

Analysis of Ingredients and DPPH, ABTS Activity for the Development of Cosmetic Raw Materials using 5 Kinds of Plants Native to Mt. Jiri.-Ⅲ

Five species, including Synurus plamatopinnatifidus (Makino) Kitam, Lindera obtusiloba Blume, Acorus gramineus Sol., Swertia japonica (Schult.) Griseb., and Valeriana fauriei Briq., native to Mt. Jiri, were collected from their habitats from April to October 2022. The collected plants were analyzed for 17 types of polyphenol components in the whole plant, and the antioxidant activities of DPPH and ABTS were measured according to the parts of the leaves and roots, and the contents of total polyphenol and total flavonoid were measured. Based on these results, we hope that they will be used as basic data for the development of antioxidant cosmetic raw materials targeting the five plants native to Mt. Jiri with high antioxidant efficacy. 1. The polyphenol components contained in the five species of plants native to Mt. Jiri were 12 types, including catechin and quercetin. Among them, the total polyphenol content of A. gramineus was 39.5ppm, and the variety with the lowest polyphenol content was S. japonica with 5.9ppm. 2. DPPH radical scavenging activity was confirmed by EC50 value. As a result, the variety with the highest DPPH radical scavenging activity was L. obtusiloba leaves at 35.31μg/mL. The variety with the lowest scavenging activity was V. fauriei at 262.69μg/mL for the leaves and 278.99μg/mL for the roots. 3. ABTS radical scavenging activity was confirmed by EC50 value. The variety with the highest scavenging activity was L. obtusiloba roots at 55.79μg/mL and 58.85μg/mL for leaves. The variety with the lowest scavenging activity was S. japonica leaves at 576.5μg/mL. 4. The variety with the highest total polyphenol content was L. obtusiloba leaves at 158.72mg/g, and the variety with the lowest content was V. fauriei leaves at 0.13mg/g. Therefore, there was a large difference in the total periphenol content by variety. 5. The variety with the highest total flavonoid content was L. obtusiloba leaves at 163.75mg GAE/g, and the other varieties showed low contents at 17.67~41.38mg GAE/g.

Five species, including Synurus plamatopinnatifidus (Makino) Kitam, Lindera obtusiloba Blume, Acorus gramineus Sol., Swertia japonica (Schult.) Griseb., and Valeriana fauriei Briq., native to Mt. Jiri, were collected from their habitats from April to October 2022. The collected plants were analyzed for 17 types of polyphenol components in the whole plant, and the antioxidant activities of DPPH and ABTS were measured according to the parts of the leaves and roots, and the contents of total polyphenol and total flavonoid were measured. Based on these results, we hope that they will be used as basic data for the development of antioxidant cosmetic raw materials targeting the five plants native to Mt. Jiri with high antioxidant efficacy. 1. The polyphenol components contained in the five species of plants native to Mt. Jiri were 12 types, including catechin and quercetin. Among them, the total polyphenol content of A. gramineus was 39.5ppm, and the variety with the lowest polyphenol content was S. japonica with 5.9ppm. 2. DPPH radical scavenging activity was confirmed by EC50 value. As a result, the variety with the highest DPPH radical scavenging activity was L. obtusiloba leaves at 35.31μg/mL. The variety with the lowest scavenging activity was V. fauriei at 262.69μg/mL for the leaves and 278.99μg/mL for the roots. 3. ABTS radical scavenging activity was confirmed by EC50 value. The variety with the highest scavenging activity was L. obtusiloba roots at 55.79μg/mL and 58.85μg/mL for leaves. The variety with the lowest scavenging activity was S. japonica leaves at 576.5μg/mL. 4. The variety with the highest total polyphenol content was L. obtusiloba leaves at 158.72mg/g, and the variety with the lowest content was V. fauriei leaves at 0.13mg/g. Therefore, there was a large difference in the total periphenol content by variety. 5. The variety with the highest total flavonoid content was L. obtusiloba leaves at 163.75mg GAE/g, and the other varieties showed low contents at 17.67~41.38mg GAE/g.