This study aims at favorable environmental conditions by river otter residing in Hongcheon river ecosystems using spraints along the river. Otter spraints were indicative of habitat use pattern and marking a territory in the areas. Nae-chon and Kuneob-chon river otter spraints were collected totaling 478 with 8 times during 2009-2011, and based on the number river use patterns were analyzed with the technique of index of dispersion. Results with larger than one indicated that river otter habitat use pattern were not random; instead they used preferred areas for habitat use. ‘I’ values greater than one indicate a clumped distribution and lower than one indicate random distribution. This study also demonstrated that we need a sophiscated linear model that should be developed to identify key habitat elements in river ecosystems.