Diphenylhydantoin이 태생생쥐의 중추신경계 기형유발에 미치는 영향

Teratogenic Effects of Diphenylhydantoin on Central Neurous System of Fetal Mouse

To elucidate the teratogenic effect of DPH to the development of central nervous system in fetal mouse, each dose of 200 mg/kg and 400 mg/kg of DPH were administered orally, on the 4th day 8th day, to respective subgroups of pregnant mice. The fetal mice were removed from uteri on the 17th day of gestation and were fixed in Bouin’ s solution, Paraffin-sectioned, and processed for microscopy. The results are summarized as follows : 1) DPH seemed to cause the increased proliferation of neuroepithelial cells during the formation of neocortex, resulting in the increased thickness of neuroepithelial layger, neocortex and the increased size of hippocampus. 2) DPH also seemed to cause the degenerative changes of neuroepithelia and neuroblasts in brain and in spinal cord, resulting in the decrease of brain parenchyma and increase of the sizes of lateral ventricle, 3rd rentricle and interventricular foramina. 3) DPH brought some structural abnormalities in the formation around lateral ventricles. 4) Administration of 200 mg/kg DPH on the 8th day did not seem to exert any visible effect to the fetuses while 400 mg/kg of DPH on the 4th day showed most massive damages among other 3 subgroups. 5) The cells of spinal cord were more susceptible to the teratogenic effects of DPH than those of the brain.