The method for determination of haloperidol in serum is based on high-performance isocratic liquid chromatography with the use of ^18 bonded reversed.phase column at room temperature. The mobile phase is a 40/60 (by Vol.) mixture of acetonitrille and 0.05M phosphate buffer (pH 4.3). Degassing is performed for 5 min. in ultrasonic cleaner. Haloperidol and the internal standard (Amitriptyline) are extracted from alkalized serum into isoamylalcohol/hexane (2/98 by Vol.) and back-extracted into HCI and then 40 ul of these samples were injected on the column. The authors used a high-performance liquid chromatography with a fixed wave length (214 nm) ultraviolet detector. A graph of ratio of the peak height of haloperidol to that of amitriptyline against drug concentration was linear and passed through the origin. The detection limit of this method was 2 ng/ml of haloperidol and the coefficient of variation at 10 ng/ml was 5.5% and at 20 ng/ml it was 10.4%. According to the results, this method appears to be suitable for monitoring haloperidol serum levels in chronic schizophrenc patients and also for the investigation of the correlation between serum levels and pharmacological effects.
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