Phenobarbital이 마우스의 酒精 代謝에 미치는 影響

Effect of Phenobarbital on the Ethanol Metabolism of Mice

An attempt was made to observe the changes in the ethanol clearance rate and the activities of enzymes involved in the metabolism of ethanol after the administration of sodium phenobarbital a well known inducer of several enzymes. The results were as follows. 1. Alcohol dehydrogenase was distributed mainly in the liver and some activities were shown in the: stomach also, while aldehyde dehydrogenase showed high activities both in the liver and in the stomach. 2. Both the activities of alcohol dehydrogenase and aldehyde dehydrogenase were negligible at the time of birth, but increased with age reaching maximum at 4 weeks after birth for the alcolhol dehydrogenase and 6 weeks for the aldehyde dehydrogenase. The increments of enzyme activities were closely related with the rate of ethanol clearance. 3. Alcolhol dehydrogenase activities both in the liver and the stomach of mice treated with sodium phenobarbital for 4 weeks did not show significant changes, while aldehyde dehydrogenase activities in the liver increased almost two-fold. 4 . The rate of ethanol clearance was increased about 20% in sodium phenobarbital-treated group, which might be due to two-fold increase of aldehyde dehydrogenase activities in the liver.