전기자극 스트레스 또는 심리갈등 스트레스가 횐쥐 비장 임파구 증식과 Interleukin-2 생산능에 미치는 여향

Effect of Electric Shock Stress or Psychological Conflict Stress on the Proliferation and Interieukin-2 Productio of Rat Splenic Lymphocyte

The purpose of this study was to examine the relstionship between stress and immunologic function as a possible mechanism of stress and disease process. The authors investigated how the psychological stress induced by avoidance-avoidance conflict model or physical stress of electric shock do impact the immune function, measuring proliferation and interleukin-2 (IL- 2) production of rat splenic lymphocytes stimulated with various mitogens such as concanavalin- A(Con-A)，phytohemagglutinin-M ( PHA) and phorbol 12-myristate 13-acetate+ calcium inophore A23187(PMA+A23187). In this experiment, female Sprag-Dawley rats were used an subjects. Animals were separated into 4 group according to different stress conditions. Conflict group received psychological conflict stress with electric shock depending on the coping behaviour and Yoked group was not in psychological conflict situation but received as same amount of electric shock as conflict group. Skinner-Box control group was immobilized without electric shock and Home-cage control group had no stress condition. The results are as follows; 1) There is significant difference in lymphocyte proliferation in case of Con-A and PMA + A 23187 between Skinner-Box control group and Yoked group at the high current condition. 2) There is significant difference in IL-2 production in case of P H A and PMA+A23187 between Skinner-Box control group and Yoked group. 3) There is more significant difference in lymphocyte proliferation in case of all of mitogens, Con-A, PHA and PM A 十 A23187 between Yoked group and Conflict group regardless of high or low current condition. 4) There is significant difference in IL-2 production in case of PMA+A23187 between Yoked group and Conflict group. This results suggest that psychological stress could reduce the immue function more profoundly than physical stress and also means the importance of coping and appraisal in the stressimmunology relationship.