3T3-L1 전지방세포에서 연잎-연근 혼합 발효물의 지방세포 분화 억제 효과
Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes
- 이신지(Sin Ji Lee) Shambhunath Bose 이수진(Su Jin Lee) 정지은(Ji Eun Jeong) 구병수(Byung Soo Koo) 김동일(Dong Il Kim) 김호준(Ho Jun Kim)
- 제13권 2호
- 등재여부 : KCI등재
- 74 - 83 (10 pages)
This study was performed to evaluate the effects of fermented lotus extracts on the inhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probiotics separately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve, and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red O dye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer binding proteins (C/EBP ) and peroxisome proliferators-activated receptor (PPAR ) were analyzed by real time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibiting preadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showed reduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibition of C/EBP . Bifidobacterium breve and Bifidobacterium longum showed the best downregulation of PPAR expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocyte differentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipid metabolism related with obesity.
재료 및 방법