의이인탕 추출방법에 따른 생리활성 연구

Study on Biological Effect of Euiiin-tang, yiyiren-tang Extracts by Extraction Methods

The purpose of this study is to investigate biological effects of Euiiin-tang(yiyiren-tang) extracts by extraction methods. Methods To investigate the effects of anti-oxidative and anti-inflammatory activity, we measured DPPH radical scavenging assay, collagenase inhibition assay and COX-2 inhibition assay. Results In case of measuring anti-oxidative and anti-inflammatory activities of Euiiin-tang(yiyiren-tang) by collagenase and COX-2 inhibition activity, it came out that Euiiin-tang(yiyiren-tang) was unable to inhibit collagenase, but was effective to inhibit COX-2. Cytotoxicity analysis of Euiiin-tang(yiyiren-tang) extracts by MTT assay principles, there was no cytotoxicity in aqueous and 50% ethanol extracts in any level of concentration. Conclusions This results suggest that Euiiin-tang(yiyiren-tang) extracts have anti-oxidative and anti-inflammatory activities.