어린 흰쥐 뇌에서 부신절제가 전기경련 충격에 의한 조기유전자 발현에 미치는 영향

The Effect of Adrenalectomy on the Induction of Immediate Early Genes by Electroconvulsive Shock in Young Rat Brain

To evaluate the influences of adrenocortical hormone on the signal transduction system in young brain, the authors observed the effects of bilateral adrenalectomy on the induction of immediate early genes(IEGs) such as c-fos, jun B, and TIS 1 by electroconvulsive shock(ECS) in the young rats. The young(70g) male Sprague-Dawley rats were treated with ECS(130V, 0.5sec) at day 3 or 14 after bilateral adrenalectomy. They were sacrificed at 0, 15，30, 45, and 60 minutes after ECS, and cerebral cortex and hippocampus were dissected from their brains. The induction of IEGs were assessed with northern hybridization. The band intensities of the autoradiographs measured by densitometer were compared between adrenalectomized group and shamoperated control group. In the cerebral cortex, c-fos and jun B appeared and disappeared earlier, and enhanced in the earlier phase of the induction in the adrenalectomized group. These trends were more prominent for jun B than for c-fos, and were more prominent at day 14 than at day 3 after adrenalectomy. The induction of TIS 1 was attenuated at day 14 after adrenalectomy. In the hippocampus, the induction of c-fos and jun B by ECS was not influenced by adrenalectomy. The induction of TIS 1 was attenuated at day 3 after adrenalectomy. These results suggest that adrenalectomy has influences on the induction of IEGs by ECS in rat brain differently, depending on the brain region, the kind of IEGs, the age of the rats, and the time passed after adrenalectomy.