Using the cerebellum of adult rats(220-24Cgmof bcdy weight), PASᅳpositive granules such as ceroids and lipofuchsin in Purkinje cells was studied histochemically and statistically. 1. PAS—positive granules present in the rat cerebellum was resistant to saliva digestion, and showed slight metachromatic reaction to the 0.5% toluidine blue aqueous solution. And no significant changes in size and number of the granules was observed, after pyridine extraction. These histochemical results may indicate that PAS-positive granules in Purkinje cells composed mainly of carbohydrate-protein plex, mucopolysaccharide or mucoprotein. 2. Number of PAS- positive granules in a Purkinje cell of the experimental groups, which had sleeping disturbance by external sti mulation for 7 days, are compared with those of control groups. Average number of PAS-positive granules ia the cell of experimental groups was 6.7 ± 0.4 , whereas that of the control groups was 8.5 ± 0 . 4. Difference of mean value calculated was statistically significant (p〈0 . 01). These results were discussed in relation to the formation and accumulation of the granules in the cells during the experimental condition.
緖 論
實驗資 및 方法
結 果
考 按
結 論
References