흰쥐 탈회 경조직의 이식시 탈회정도에 따른 조직반응에 관한 연구

The histochemical and ultrastructural feature of tissue reaction was studied after implantation of decalcified hard tissues which were demineralized completely or partially in 0.6 N HC1. Decalcified tooth, parietal bone, and femur of rat were implanted on rat epimyslum. The completely* or partially- decalcified tissue implants caused different tissue reaction around/in the implant. Partially decalcified hard tissues were surrounded with vascular connective tissue in which no cartilage or bone was developed. The most characteristic feature of cells adjacent to partially decalcified implants was the numerous mast cells were found near the implants and a number of macrophages contacting it. The cartilage and bone were developed in the case of the implantation of the completely decalcified hard tissue. The most cartilage was resorbed at the time of bone development, but a part of cartilage matrix was found in the matrix of new bone. Recalcified implants not related with cartilage or bone development were found among completely decalcified implants. These recalcified implants were surrounded with osteoclast-like giant cells, which suggested that the exposed calcified matrix is important in the differentiation of osteoclast-like cells.