동결보호제 첨가에 따른 ？？동 보관 자가골 이식후 초기골 재생에 관한 연구

Purpose : Autogenous bone has been reported the most effective materials but it has disadvantage of difficulties in preservation. Cryopreservation is one of the method but simply freezing brings about cell necrosis which affect the result of bone graft. Accordingly, for the research of the ciyopreservative effect on bone regeneration after replantation of freezed autogenous bone, animal study was done and this is the report of preliminary result. Materials and Methods : Six rabbits were used as experimental animals. Surgical defects were created on the distal femoral heads and mesial tibial heads of each animal using trephine drill.(5mm diameter) the harvested bones were cryopreserved with/without cryoprotectant in-80 C refrigerator for one week, the defects were filled with cryopreserved bone with/without cryoprotectants as experimental group and control group. Then, the animals were sacrified at 1,2 and 3 weeks after surgery. With Goldner’ s modified Masson trichrome stainign and semiautomatic image analysis system, I observed the change of the cells and bone formation Results : After bone graft, bone formation and active remdeling prosess were examined in all experimental groups and the control. But the intensity of such activities of EG(-) group were somewhat weaker than that of Control group and EG(+) group. Control group was the best in bone formation and bone remodeling. Especially EG(+) groupresembled in histologic findings and was observed of bony callus. Histologic findings showed superior bony quantity and quality in EG(+) group than that in EG(-)group