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Vanadate가 골격근 sarcoplasmic reticulum의 Ca<sup>++</sup>-ATPase 및 Ca<sup>++</sup> uptake에 미치는 영향

Effects of Vanadate on Activity of Ca<sup>++</sup>Activated ATPase and Ca<sup>++</sup> Uptake of Sarcoplasmic Reticulum in Rat Skeletal Muscle

Since it has been reported that vanadate inhibits Ca<sup>++</sup>-ATPase activity without affecting Ca<sup>++</sup> uptake, this study was undertaken to investigate the effects of vanadate on Ca<sup>++</sup>-ATPase activity and Ca<sup>++</sup> uptake in the sarcoplasmic reticulum of rat skeletal muscle. The following results were obtained. 1) Ca<sup>++</sup> activated ATPase activity of the intact sarcoplasmic reticulum was significantly inhibited when vanadate was added to the incubation medium at concentration greater than 10<sup>-6</sup> M. However Mg<sup>++</sup>-ATPase activity of the intact SR was not affected by vanadate at concentrations ranging from 10<sup>-7</sup> to 10<sup>-4</sup> M. Similarly, Ca<sup>++</sup>-ATPase activity in sonicated sarcoplasmic reticulum was significantly reduced by vanadate at a concentration 10<sup>-7</sup> M or higher. 2) The uptake of Ca<sup>++</sup> by isolated sarcoplasmic reticulum was also inhibited by vanadate under the conditions where the turnover rate of Ca<sup>++</sup>-ATPase was made to increase. These results suggest that the inhibition of Ca<sup>++</sup> uptake by vanadate may be correlated with that of Ca<sup>++</sup>-ATPase if experimental conditions are properly set.

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