고려인삼이 ACTH를 받은 마우스의 악하선 조직 DNA 합성능에 미치는 영향( II )

Influence of Panax Ginseng on DNA Synthesis of Submandibular Gland in Mice Receiving ACTH(II)

It was planned to investigate, in mice that received ACTH, the influence of Panax Ginseng upon DNA synthesis of submandibular gland by observing incorporation of [³H] thymidine into the tissue cells. Thirty male mice (body weight: 18 ~ 20 g) were divided equally into the ginseng-ACTIH and the saline-ACTH groups. Each animal of the ginseng-ACTH and the saline-ACTH groups received every day (subcutaneously) 0.05 m1/10 g body weight of ginseng extract(4 mg of ginseng alcohol extract in 1 ml of saline) and the same amount of saline, respectively, for 5 days. On the 5th experimental day, all animals received 0.01 unit of ACTH intraperitoneally one hour after the last medication, and 1 μCi/g body weight of [³H] thymidine after one more hour. Five animals, at a time, of each group were sacrificed 1, 10, and 24 hr after [³H] thymidine administration, and the radioactivity of cells in their mandibular gland was measured autoradiographically in terms of the % number of radioactive cells in 1,000 cell counts (Radioactive Index, R.1.). Results obtained were as follows: 1. The radioactive indices obtained from submandibular gland of the saline-ACTH group 1, 10 and 24 hr after [³H] thymidine administration were 15.2±0.32, 20.1±0.30, and 24.5±0.52(mean±S.D.) in the mucous cells, 13.0±0.22, 10.2±0.05, and 7.5±0.42 in the serous cells. and 10.5±0.40, 13.6±0.32, and 15.9±0.42 in the duct cells, while the mean±S.D. of the values obtained from the 3 cell types 1, 10 and 24 hr after [³H] thymidine were 10.9±0.28, 12.4±0.31, and 10.0±0.39. Thus the radioactive indices obtained from the ginseng-ACTH group were generally lower than those obtained from the saline-ACTH group. It is inferred from the above results that the ginseng tends to promote the suppressive action of ACTH upon DNA synthesis of cells in the mandibular gland.