동방결절 전기적 특성에 대한 Ca<sup>2+</sup> 효과의 온도에 따른 변화
Temperature-dependency of Ca<sup>2+(/sup) Effect on the Electrical Activity of Rabbit SA Node
- 호원경(Ho, Won-Kyung) 김기환(Kim, Ki-Whan) 황상익(Hwang, Sang-Ik)
- 대한생리학회
- 대한생리학회지
- 제21권 제1호
- 1987.06
- 1 - 12 (12 pages)
There is evidence that the effect of extracellular Ca<sup>2+</sup> on heart rate is temperature-dependent: at 38℃ excess Ca<sup>2+</sup> induces positive chronotropic response, whereas at 30℃ there is no significant chronotropic effect of Ca<sup>2+</sup>. The cause of this temperature-dependency, however, remains still unclear. Therefore, this study was undertaken to investigate the chronotropic effect of external Ca<sup>2+</sup> at different temperature in the isolated rabbit atria and in the small strips of SA node cut perpendicularly to crista terminalis. In the isolated atria, the Ca<sup>2+</sup> effect was temperature-dependent: at 35℃ excess Ca<sup>2+</sup> evoked positive chronotropic response, while at 30℃ there was no significant changes in sinus rate. On the contrary, in the small SA strips external Ca<sup>2+</sup> induced negative chronotropic effect. At 35℃ changes in Ca<sup>2+</sup> concentration from 2 to 4, 6, and 10 mM decreased the sinus rate by 2.7±1.6%, 11.2±3.7% and 23.2±8.1% respectively. Lowering the temperature to 30℃, the negative chronotropic effect of Ca<sup>2+</sup> became greater. With intracellular microelectrodes transmembrane potential was recorded in the small SA strips at 30℃, 35℃ and 38℃. As temperature increased from 30 to 38℃, sinus rate was accelerated by 13/min/℃, APD<sub>50</sub>(action ptential duration from peak to 50% repolarization) decreased by 5 msec/℃, and amplitude of action potential was slightly decreased. With an increase in Ca<sup>2+</sup> concentrations from 0.5 to 6 mM, overshoot increased and MDP decreased. These Ca<sup>2+</sup> effects on the overshoot and MDP of action potentials were not altered by temperature. But the Ca<sup>2+</sup> effects on the rates of diastolic depolarization, systolic depolarization and repolarization were modified by temperature. Discrpancy of the chronotropic effects of Ca<sup>2+</sup> between isolated atria and small SA strips was discussed.