흰쥐의 적출한 위(胃)에서 산증(酸症)과 Prostaglandin E<sub>1</sub>이 위산(胃酸) 분비에 미치는 영향
Effect of Acidosis and Prostaglandin E<sub>1</sub> upon Acid Secretion in Isolated Whole Stomach in Rat
- 김철(Kim, Chul) 김정진(Kim, Chung-Chin) 박형진(Park, Hyoung-Jin)
- 대한생리학회
- 대한생리학회지
- 제14권 제2호
- 1980.12
- 17 - 20 (4 pages)
This study was undertaken to investigate the effect of the acidosis on the gastric acid secretion in the isolated whole stomach of the rat and the effect of prostaglandin E<sub>1</sub> on the gastric acid secretion influenced by the acidosis. Twenty-two male albino rats(Sprague-Dawley strain) were used. The isolated whole stomach from each rat was introduced into the Kreb s solution which was continuously gassed with 95%O<sub>2</sub>-5%CO<sub>2</sub> for 1 hour, after irrigation of the lumen with cold physiological saline(4℃). Thereafter, each stomach was irrigated again with 5% dextrose solution (pH 7.4, 37℃), and filled with the dextrose solution. All the stomachs with the dextrose solution were divided into 4 groups according to the Kreb s solutions in which each stomach was incubated for 30 min: 1) control group, in the pH 7.4 solution, 2) PGE<sub>1</sub> group, in the pH 7.4 solution containing 5 μg/ml of PGE<sub>1</sub>, 3) acid group, in the pH 7.0 solution, and 4) acid+PGE<sub>1</sub> group, in the pH 7.0 solution containing 5 μg/ml of PGE<sub>1</sub>. After incubatory period, the contents of each stomach were collected and centrifuged(1,500 rpm, room temperature) for 15 min. The acid output in the supernatant was determined with 0.012 N NaOH by means of autotitrator(Dosimat, Metrohm Herisau Co.) at pH 7.4. Results obtained were as follows: 1) The acid output of the acid group increased significantly in comparison with the control value. 2) The acid output of the acid+PGE<sub>1</sub> group decreased significantly in comparison with the acid group. It is inferred from the above results that the acidosis facilitates the gastric acid secretion and PGE<sub>1</sub> inhibits the gastric acid secretion induced by the acidosis.