L-Glutamate에 의한 PC12 세포의 고사성 사망
Apoptotic Process is Involved in the L-Glutamate-Induced PC12 Cell Death
- 성기욱(Ki Wug Sung) 정경희(Kyung Heui Jung) 김성윤(Seong Yun Kim) 강정혜(Jung Hyae Kang) 이상복(Sang Bok Lee)
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제1권 제6호
- 등재여부 : KCI등재
- 1997.01
- 699 - 705 (7 pages)
<P> Although it is known that neuronal cell death during development occurs by apoptosis, the mechanisms underlying excitatory amino acid-induced neuronal cell death remain poorly understood. In this study we have examined the mechanism by which L-glutamate, an excitatory amino acid neurotransmitter, induces cell death in PC12 cell lines. <P> To characterize cell death, we employed sandwich enzyme-linked immunosorbent assay(ELISA) method for cellular DNA fragmentation, DNA agarose gel electrophoresis and chromatin staining by acridine orange and ethidium bromide after treating the PC12 cells with L-glutamate. L-Glutamate caused dose-dependent cell death with a maximum at 24 hrs after the treatment. These cellular fragmentation was blocked by pretreatment of MK-801, a noncompetitive <I>N</I>-methyl-D-aspartic acid(NMDA) receptor antagonist, and nerve growth factor(NGF). Analysis of DNA integrity from L-glutamate-treated cells revealed cleavage of DNA into regular sized fragments, a biochemical hallmark of apoptosis. The PC12 cells that were induced to die by L-glutamate treatment exhibited classical chromatin condensation under the light microscopy after acridine orange and ethidium bromide staining. <P>These results suggest that apoptosis is one of the key features that are involved in L-glutamate-induced excitotoxic cell death in PC12 cells, and these cell death are mediated by NMDA receptor and depend on NGF.