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KCI등재 학술저널

해마절편의 허혈성 K+ 축적에 대한 K+채널 조절 약물의 작용

Effects of K+ Channel Modulators on Extracellular K+ Accumulation during Ischemia in the Rat Hippocampal Slice

<P> Loss of synaptic transmission and accumulation of extracellular K<SUP>&#8290;</SUP>([K<SUP>&#8290;</SUP>]<SUB>O</SUB>) are the key features in ischemic brain damage. Here, we examined the effects of several K<SUP>&#8290;</SUP>channel modulators on the early ischemic changes in population spike (PS) and [K<SUP>&#8290;</SUP>]<SUB>o</SUB> in the CA1 pyramidal layer of the rat hippocampal slice using electrophysiological techniques. After onset of anoxic aglycemia (AA), orthodromic field potentials decreased and disappeared in 3.3&#8273;0.22 min (mean&#8273;SEM, n=40). The hypoxic injury potential (HIP), a transient recovery of PS appeared at 6.0&#8273;0.25 min (n=40) in most slices during AA and lasted for 3.3&#8273;0.43 min. [K<SUP>&#8290;</SUP>]<SUB>O</SUB> increased initially at a rate of 0.43 mM/min (Phase 1) and later at a much faster rate (12.45 mM/min, Phase 2). The beginning of Phase 2 was invariably coincided with the disappearance of HIP. Among K<SUP>&#8290; </SUP>channel modulators tested such as 4-aminopyridine (0.03, 0.3 mM), tetraethylammonium (0.1 mM), NS1619 (0.3∼10 &#1356;M), niflumic acid (0.1 mM), glibenclamide (40 &#1356;M), tolbutamide (300 &#1356;M) and pinacidil (100 &#1356;M), only 4-aminopyridine (0.3 mM) induced slight increase of [K<SUP>&#8290;</SUP>]<SUB>O</SUB> during Phase 1. However, none of the above agents modulated the pattern of Phase 2 in [K<SUP>&#8290;</SUP>]<SUB>O </SUB>in response to AA<SUB>.</SUB> Taken together, the experimental data suggest that 4-aminopyridine-sensitive K<SUP>&#8290;</SUP>channels, large conductance Ca<SUP>2&#8290;</SUP>-activated K<SUP>&#8290;</SUP> channels and ATP-sensitive K<SUP>&#8290;</SUP> channels may not be the major contributors to the sudden increase of [K<SUP>&#8290;</SUP>]<SUB>O </SUB>during the early stage of brain ischemia, suggesting the presence of other routes of K<SUP>&#8290;</SUP>efflux during brain ischemia.

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