The Inhibitory Effect of Eupatilin on <I>Helicobacter Pylori</I>-Induced Release of Leukotriene D<SUB>4</SUB> in the Human Neutrophils and Gastric Mucosal Cells

The Inhibitory Effect of Eupatilin on <I>Helicobacter Pylori</I>-Induced Release of Leukotriene D<SUB>4</SUB> in the Human Neutrophils and Gastric Mucosal Cells

<P> In this report, the inhibitory action of eupatilin was investgated by using leukotriene D<SUB>4</SUB> in the human neutrophils and Kato III cells (Gastric adenoma cells as a substitute for gastric mucosal cells) stimulated by <I>Helicobacter pylori.</I> Leukotriene D<SUB>4 </SUB>(LTD<SUB>4</SUB>) was released from both neutrophils and Kato III cells when these cells were incubated with <I>H. pylori</I>. The release of LTD<SUB>4</SUB> increased time-dependently and the maximum release of LTD<SUB>4 </SUB>was 2.3∼2.5 pmol. But in the presence of eupatilin, the release of LTD<SUB>4</SUB> from these cells was inhibited in a dose-dependent manner. In the neutrophils, the release of LTD<SUB>4 </SUB>was suppressed to 70% and 50% of the control levels when neutrophils was incubated with 0.01 and 0.1 mM of eupatilin. In the Kato III cells, the release of LTD<SUB>4</SUB> was suppressed to 59% and 27% of the control levels by adding 0.01 and 0.1 mM of eupatilin. We estimated the intracellular Ca<SUP>2&#8290; </SUP>levels when Kato III cells and neutrophils were stimulated by <I>H. pylori </I>using <SUP>45</SUP>Ca. But the suppressive effect of eupatilin on Ca<SUP>2&#8290;</SUP> influx into these cells was not significant. We also obtained the results that <I>H. pylori</I> induced Ca<SUP>2&#8290;</SUP> influx into these cells by confocal microscopy, however there was no differences in the dose level of eupatilin. These results were confirmed by <SUP>1</SUP>H Nuclear Magnetic Resonance(NMR) spectroscopy. The NMR patterns of eupatilin in the absence of Ca<SUP>2&#8290;</SUP> was changed compare with when Ca<SUP>2&#8290;</SUP> was present, but its effect was not strong.