Mouse Leukemia 세포에서 Adenosine 5’-triphosphate에 의 한 Apoptosis

Apoptosis Induced by Adenosine 5’-triphosphate in Mouse Leukemic Cells.

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Extracellular ATP elicits various biological responses and plays a significant role in physiological regulation. Recent1y， ATP-induced growth inhibitions were reported in some tumor cell lines， but these effects and mechanisms are not well known. This study was conducted to investigate ATP-induced growth inhibition in mouse leukemic(P388DJ) cells. ATP inhibited cell growth in a dose-dependent manner as analyzed by MTS assay(IC5o: 33.1 μM). Nuc1eotides other than ATP， such as ADP(37.5 μM) and AMP(33.2 μM) had the same effects as ATP，but adenosine(57.8 μM) showed less effect than ATP. ATP attenuated the cells in Go/GJ and G2/M phases，but increased those in S phase in flow cytometric analysis. Hypodiploid cells(Ao)， the presumptive findings of apoptosis， were found among the ATP-treated cells. ATP induced DNA fragmentation into 180-200 bps as measured by electrophoresis. Some apoptotic cells were stained by TUNEL method. ATP increased the intracellular++ ... ， .... .--++ free Ca concentration([Ca]i) and the increment of [Ca]i was caused by influx from the extracellu1ar space. These results suggest that extracellular ATP induces growth inhibition through apoptosis.