Effects of Ethanol on Na<SUP>⁢</SUP>-dependent Solute Uptake in Rabbit Renal Brush-Border Membrane Vesicles
Effects of Ethanol on Na<SUP>⁢</SUP>-dependent Solute Uptake in Rabbit Renal Brush-Border Membrane Vesicles
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제3권 제2호
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1999.01191 - 198 (8 pages)
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<P> This study was undertaken to examine the effect of ethanol on Na<SUP>⁢</SUP>-dependent transport systems (glucose, phosphate, and dicarboxylate) in renal brush-border membrane vesicles (BBMV). Ethanol inhibited Na<SUP>⁢</SUP>- dependent uptakes of glucose, phosphate, and succinate in a dose-dependent manner, but not the uptakes of Na<SUP>⁢</SUP>-independent. The H<SUP>⁢</SUP>/TEA antiport was reduced by 8% ethanol. Kinetic analysis showed that ethanol caused a decrease in V<SUB>max</SUB> of three transport systems, leaving K<SUB>m</SUB> values unchanged. Ethanol decreased phlorizin binding, which was closely correlated with the decrease in V<SUB>max</SUB> of Na<SUP>⁢</SUP>-glucose uptake. These results indicate that ethanol inhibits Na<SUP>⁢</SUP>-dependent uptakes of glucose, phosphate, and dicaboxylate and that the reduction in V<SUB>max </SUB>of Na<SUP>⁢</SUP>-glucose uptake is caused by a decrease in the number of active carrier proteins in the membrane.
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