Magnesium Suppresses the Responses of Dorsal Horn Cell to Noxious Stimuli in the Rat

Magnesium Suppresses the Responses of Dorsal Horn Cell to Noxious Stimuli in the Rat

<P> Magnesium ion is known to selectively block the N-methyl-D-aspartate (NMDA)-induced responses and to have anticonvulsive action, neuroprotective effect and antinociceptive action in the behavioral test. In this study, we investigated the effect of Mg<SUP>2&#8290;</SUP> on the responses of dorsal horn neurons to cutaneous thermal stimulation and graded electrical stimulation of afferent nerves as well as to excitatory amino acids and also elucidated whether the actions of Ca<SUP>2&#8290;</SUP> and Mg<SUP>2&#8290;</SUP> are additive or antagonistic. Mg<SUP>2&#8290;</SUP> suppressed the thermal and C-fiber responses of wide dynamic range (WDR) cell without any effect on the A-fiber responses. When Mg<SUP>2&#8290;</SUP> was directly applied onto the spinal cord, its inhibitory effect was dependent on the concentration of Mg<SUP>2&#8290;</SUP> and duration of application. The NMDA- and kainate-induced responses of WDR cell were suppressed by Mg<SUP>2&#8290;</SUP>, the NMDA-induced responses being inhibited more strongly. Ca<SUP>2&#8290;</SUP> also inhibited the NMDA-induced responses current-dependently. Both inhibitory actions of Mg<SUP>2&#8290;</SUP> and Ca<SUP>2&#8290;</SUP> were additive, while Mg<SUP>2&#8290;</SUP> suppressed the EGTA-induced augmentation of WDR cell responses to NMDA and C-fiber stimulation. Magnesium had dual effects on the spontaneous activities of WDR cell. These experimental findings suggest that Mg<SUP>2&#8290;</SUP> is implicated in the modulation of pain in the rat spinal cord by inhibiting the responses of WDR cell to noxious stimuli more strongly than innocuous stimuli.