Ceramide is involved in MPP<SUP>⁢</SUP>-induced Cytotoxicity in Human Neuroblastoma Cells
Ceramide is involved in MPP<SUP>⁢</SUP>-induced Cytotoxicity in Human Neuroblastoma Cells
- Eun-Joo Nam Hye-Sook Lee Young Jae Lee Wan-Seok Joo Sungho Maeng Hye-In Im Chan-Woong Park Yong Sik
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제6권 제6호
- 등재여부 : KCI등재
- 2002.01
- 281 - 286 (6 pages)
<P> To understand the cytotoxic mechanism of MPP<SUP>⁢</SUP>, we examined the involvement of ceramide in MPP<SUP>⁢</SUP>- induced cytotoxicity to human neuroblastoma SH-SY5Y cells. When SH-SY5Y cells were exposed to MPP<SUP>⁢</SUP>, MPP<SUP>⁢</SUP> induced dose-dependent cytotoxicity accompanied by 2-fold elevation of intracellular ceramide levels in SH-SY5Y cells. Three methods were used to test the hypothesis that the elevated intracellular ceramide is related to MPP<SUP>⁢</SUP>-induced cytotoxicity: C<SUB>2</SUB>-ceramide was directly applied to cells, sphingomyelinase (SMase) was exogenously added, and oleoylethanolamine (OE) was used to inhibit degradation of ceramide. Furthermore, inhibition of ceramide-activated protein phosphatase (CAPP), the effector of ceramide, using okadaic acid (OA) attenuated cell death but treatment of fumonisin B<SUB>1</SUB>, the ceramide synthase inhibitor, did not alter the cytotoxic effect of MPP<SUP>⁢</SUP>. Based on these, we suggest that the elevation of intracellular ceramide is one of the important mediators in MPP<SUP>⁢</SUP>- induced cell death.