Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas
Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas
- Hyung Seo Park Se Hoon Kim Hyoung Jin Park Mee Young Lee Young Hee Han
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제7권 제4호
- 등재여부 : KCI등재
- 2003.01
- 217 - 221 (5 pages)
To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-17β on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-17β did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-17β failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-17β did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-17β. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic short- term action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.