Enhancement of ATP-induced Currents by Phospholipase D1 Overexpressed in PC12 Cells
Enhancement of ATP-induced Currents by Phospholipase D1 Overexpressed in PC12 Cells
- Jin Bong Park Young Rae Kim Byeong Hwa Jeon Seung-Kiel Park Sae-Ock Oh Young Geun Kim Sang Do Lee Kw
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제7권 제4호
- 등재여부 : KCI등재
- 2003.01
- 223 - 229 (7 pages)
Using phospholipase D1 (PLD1)-overexpressing PC12 (PLD1-PC12) cells, the regulatory roles of PLD1 on ATP-induced currents were investigated. In control and PLD1-PC12 cells, ATP increased PLD activity in an external Ca<SUP>2⁢</SUP> dependent manner. PLD activity stimulated by ATP was substantially larger in PLD1-PC12 cells than in control cells. In whole-cell voltage-clamp mode, ATP induced transient inward and outward currents. The outward currents inhibited by TEA or charybdotoxin were significantly larger in PLD1-PC12 cells than in control cells. The inward currents known as Ca<SUP>2⁢</SUP> permeable nonselective cation currents were also larger in PLD1-PC12 cells than in control cells. However, the difference between the two groups of cells disappeared in Ca<SUP>2⁢</SUP>-free external solution, where ATP did not activate PLD. Finally, ATP-induced <SUP>45</SUP>Ca uptakes were also larger in PLD1-PC12 cells than in control cells. These results suggest that PLD enhances ATP-induced Ca<SUP>2⁢</SUP> influx via Ca<SUP>2⁢</SUP> permeable nonselective cation channels and increases subsequent Ca<SUP>2⁢</SUP>-activated K<SUP>⁢</SUP> currents in PC12 cells.