<I>Vibrio vulnificus</I> Cytolysin Forms Anion-selective Pores on the CPAE Cells, a Pulmonary Endothelial Cell Line
<I>Vibrio vulnificus</I> Cytolysin Forms Anion-selective Pores on the CPAE Cells, a Pulmonary Endothelial Cell Line
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제8권 제5호
-
2004.01259 - 264 (6 pages)
- 0
Cytolysin produced by <I>Vibrio vulnificus</I> has been incriminated as one of the important virulence determinants in <I>V. vulnificus</I> infection. Ion selectivity of cytolysin-induced pores was examined in a CPAE cell, a cell line of pulmonary endothelial cell, using inside-out patch clamp techniques. In symmetrical NaCl concentration (140 mM), intracellular or extracellular application of cytolysin formed ion-permeable pores with a single channel conductance of 37.5⁑4.0 pS. The pore currents were consistently maintained after washout of cytolysin. Replacement of Na<SUP>⁢</SUP> in bath solution with monovalent ions (K<SUP>⁢</SUP>, Cs<SUP>⁢</SUP> or TEA<SUP>⁢</SUP>) or with divalent ions (Mg<SUP>2⁢</SUP>, Ca<SUP>2⁢</SUP>) did not affect the pore currents.<FONT COLOR=BLUE><SPAN STYLE= font-size:11pt; > </SPAN>When the NaCl concentration in bath solution was lowered from 140 to 60 and 20 mM, the reversal potential shifted from 0 to ⁣11.8 and ⁣28.2 mV, respectively. The relative permeability of the cytolysin pores to anions measured at ⁣40 mV was Cl<SUP>⁣</SUP> = NO<SUB>2</SUB><SUP>⁣</SUP> ≥ Br<SUP>⁣</SUP> = I<SUP>⁣</SUP> ＞ SCN<SUP>⁣</SUP> ＞ acetate<SUP>⁣</SUP> ＞ isethionate<SUP>⁣</SUP> ＞ ascorbic acid<SUP>⁣</SUP> ＞ EDTA<SUP>2⁣</SUP>, in descending order. The cytolysin-induced pore current was blocked by Cl<SUP>⁣</SUP> channel blockers or nucleotides. These results indicate that <I>V. vulnificus</I> cytolysin forms anion-selective pores in CPAE cells.
(0)
(0)