Effect of Extracts from Safflower Seeds on Osteoblastic Differentiation and Intracellular Free Calcium Concentration in MC3T3-E1 Cells
Effect of Extracts from Safflower Seeds on Osteoblastic Differentiation and Intracellular Free Calcium Concentration in MC3T3-E1 Cells
- Hye-Ock Jang Hyun-Sup Eom Sung-Bae Roh Il Yun
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제9권 제1호
- 등재여부 : KCI등재
- 2005.01
- 55 - 62 (8 pages)
Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, 3μl of 0.1% dried crude extract or 2μl of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> of the cultured osteoblast cells: 3μl of 0.1% dried crude extract and 2μl of 0.1% dried aqueous fraction significantly increased the [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> of the cultured osteoblast cells (8⁓10<SUP>4</SUP>) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> of the cultured osteoblast cells, and 300μM Cd<SUP>2⁢</SUP>, specific calcium channel blocker, completely blocked the increase. Therefore, the increased [Ca<SUP>2⁢</SUP>]<SUB>i</SUB> of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.