5-Hydroxytryptamine Inhibits Glutamatergic Synaptic Transmission in Rat Corticostriatal Brain Slice
5-Hydroxytryptamine Inhibits Glutamatergic Synaptic Transmission in Rat Corticostriatal Brain Slice
- Hyeong Seok Cho Se Joon Choi Ki-Jung Kim Hyun Ho Lee Seong Yun Kim Young Jin Cho Ki-Wug Sung
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제9권 제5호
- 등재여부 : KCI등재
- 2005.01
- 255 - 262 (8 pages)
Striatum is involved in the control of movement and habitual memory. It receives glutamatergic input from wide area of the cerebral cortex as well as an extensive serotonergic (5-hydroxytryptamine, 5-HT) input from the raphe nuclei. In our study, the effects of 5-HT on synaptic transmission were studied in the rat corticostriatal brain slice using in vitro whole-cell recording technique. 5-HT inhibited the amplitude as well as frequency of spontaneous excitatory postsynaptic currents (sEPSC) significantly, and neither γ-aminobutyric acid(GABA)<SUB>A</SUB> receptor antagonist bicuculline (BIC), nor N-methyl-<SUB>D</SUB>-aspartate (NMDA) receptor antagonist, <SUB>DL</SUB>-2-amino-5-phosphonovaleric acid (AP-V) could block the effect of 5-HT. In the presence non-NMDA receptor antagonist, 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenxo[f] quinoxaline-7-sulfonamide (NBQX), the inhibitory effect of 5-HT was blocked. We also figured out that 5-HT change the channel kinetics of the sEPSC. There was a significant increase in the rise time during the 5-HT application. Our results suggest that 5-HT has an effect on both pre- and postsynaptic site with decreasing neurotransmitter release probability of glutamate and decreasing the sensitivity to glutamate by increasing the rise time of non-NMDA receptor mediated synaptic transmission in the corticostriatal synapses.