Effects of TGF-<FONT FACE= HJ중명조 >Ղ1 Ribbon Antisense on CCl<SUB>4</SUB>-induced Liver Fibrosis
Effects of TGF-<FONT FACE= HJ중명조 >Ղ1 Ribbon Antisense on CCl<SUB>4</SUB>-induced Liver Fibrosis
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제12권 제1호
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2008.011 - 6 (6 pages)
- 0
Ribbon-type antisense oligonucleotide to TGF-<FONT FACE= HJ중명조 >Ղ1 (TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by CCl<sub>4</sub> injection. When Hepa1c1c7 cells were transfected with TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS, the level of TGF-<FONT FACE= HJ중명조 >Ղ1 mRNA was effectively reduced. TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-<FONT FACE= HJ중명조 >Ղ1 mRNA levels were significantly reduced only in the TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS-treated group. The results of immunohistochemical studies showed that TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS prevented the accumulation of collagen and <FONT FACE= HJ중명조 >Ձ-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-<FONT FACE= HJ중명조 >Ղ1 with efficient uptake can effectively prevent fibrosis of the liver.
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