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KCI등재 학술저널

Lysophosphatidylcholine Increases Ca<SUP>2+</SUP> Current via Activation of Protein Kinase C in Rabbit Portal Vein Smooth Muscle Cells

Lysophosphatidylcholine Increases Ca<SUP>2+</SUP> Current via Activation of Protein Kinase C in Rabbit Portal Vein Smooth Muscle Cells

Lysophosphatidylcholine (LPC), a metabolite of membrane phospholipids by phospholipase A<sub>2</sub>, has been considered responsible for the development of abnormal vascular reactivity during atherosclerosis. Ca<sup>2+</sup> influx was shown to be augmented in atherosclerotic artery which might be responsible for abnormal vascular reactivity. However, the mechanism underlying Ca<sup>2+</sup> influx change in atherosclerotic artery remains undetermined. The purpose of the present study was to examine the effects of LPC on L-type Ca<sup>2+</sup> current (I<sub>Ca(L)</sub>) activity and to elucidate the mechanism of LPC-induced change of I<sub>Ca(L)</sub> in rabbit portal vein smooth muscle cells using whole cell patch clamp. Extracellular application of LPC increased I<sub>Ca(L)</sub> through whole test potentials, and this effect was readily reversed by washout. Steady state voltage dependency of activation or inactivation properties of I<sub>Ca(L)</sub> was not significantly changed by LPC. Staurosporine (100 nM) or chelerythrine (3&#1356;M), which is a potent inhibitor of PKC, significantly decreased basal I<sub>Ca(L)</sub>, and LPC-induced increase of I<sub>Ca(L)</sub> was significantly suppressed in the presence of PKC inhibitors. On the other hand, application of PMA, an activator of PKC, increased basal I<sub>Ca(L)</sub> significantly, and LPC-induced enhancement of I<sub>Ca(L)</sub> was abolished by pretreatment of the cells with PMA. These findings suggest that LPC increased I<sub>Ca(L)</sub> in vascular smooth muscle cells by a pathway that involves PKC, and that LPC-induced increase of I<sub>Ca(L)</sub> might be, at least in part, responsible for increased Ca<sup>2+</sup> influx in atherosclerotic artery.

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