Voltage-dependent Ca<SUP>2+</SUP> Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
Voltage-dependent Ca<SUP>2+</SUP> Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
- Young Chul Kim Hikaru Suzuki Wen-Xie Xu Hikaru Hashitani Woong Choi Hyo-Yung Yun Seon-Mee Park Sei J
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제12권 제6호
- 등재여부 : KCI등재
- 2008.01
- 323 - 330 (8 pages)
The properties of voltage dependent Ca<sup>2+</sup> current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c<i>-Kit</i> positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min<sup>−1</sup>, respectively, in the presence of 1</SUP>ՌM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c<i>-Kit</i> immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K<sup>+</sup>-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs<sup>+</sup>-rich pipette solution at <i>V</i><sub>h</sub>=−80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1ՌM) blocked VDIC. Therefore, we successfully isolated c<i>-Kit</i> positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca<sup>2+</sup> currents (VDCC<sub>L</sub>).