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KCI등재 학술저널

Voltage-dependent Ca<SUP>2+</SUP> Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach

Voltage-dependent Ca<SUP>2+</SUP> Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach

The properties of voltage dependent Ca<sup>2+</sup> current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c<i>-Kit</i> positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min<sup>&#8722;1</sup>, respectively, in the presence of 1&lt;/SUP&gt;&#1356;M nifedipine. Single ICC-MY isolated by enzyme treatment also showed c<i>-Kit</i> immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K<sup>+</sup>-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs<sup>+</sup>-rich pipette solution at <i>V</i><sub>h</sub>=&#8722;80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1&#1356;M) blocked VDIC. Therefore, we successfully isolated c<i>-Kit</i> positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca<sup>2+</sup> currents (VDCC<sub>L</sub>).

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