Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients
Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제12권 제6호
-
2008.01337 - 342 (6 pages)
- 0
Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10<sup>th</sup> passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K<sup>＋</sup> currents, including noise-like Ca<sup>＋2</sup>-activated K<sup>＋</sup> current (IK<sub>Ca</sub>), a transient outward K<sup>＋</sup> current (I<sub>to</sub>), a delayed rectifier K<sup>＋</sup> current (IK<sub>DR</sub>), and an inward-rectifier K<sup>＋</sup> current (K<sub>ir</sub>) were heterogeneously present in these cells, and a TTX-sensitive Na<sup>＋</sup> current (I<sub>Na,TTX</sub>) was also recorded. In the RT-PCR analysis, <i>Kv1.1, heag1, Kv4.2, Kir2.1, MaxiK,</i> and <i>hNE-Na</i> were detected. In particular, I<sub>Na,TTX</sub> showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs
(0)
(0)