Water Extract of Samultang Reduces Apoptotic Cell Death by H<SUB>2</SUB>O<SUB>2</SUB>-Induced Oxidative Injury in SK-N-MC Cells
Water Extract of Samultang Reduces Apoptotic Cell Death by H<SUB>2</SUB>O<SUB>2</SUB>-Induced Oxidative Injury in SK-N-MC Cells
- Gyoung Wan Lee Min Sun Kim
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제13권 제3호
- 등재여부 : KCI등재
- 2009.01
- 139 - 145 (7 pages)
The purpose of this study was to evaluate the effects of the water extract of Samultang (SMT), a Chinese herb, on apoptotic cell death by H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in SK-N-MC cells. A nuclear fragmentation was observed via fluorescence imaging 12 h after exposure to 30</SUB>ՌM H<sub>2</sub>O<sub>2</sub> and DNA laddering was detected via agarose electrophoresis gel. In addition, increases in sub-G1 phase and cleavage of the PARP protein were observed. However, treatment with SMT for 2 h prior to H<sub>2</sub>O<sub>2</sub> exposure significantly reduced apoptotic cell death induced by incubation with 30ՌM H<sub>2</sub>O<sub>2</sub> in SK-N-MC cells. Pre-incubation with water extract of SMT for 2 h prevented the H<sub>2</sub>O<sub>2</sub>-induced decrease in mitochondrial transmembrane potential. SMT also attenuated the increase in caspase-3 activity and the breakdown of PARP protein caused by H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. These results suggest that the water extract of SMT provides inhibition of apoptotic cell death against oxidative injury in SK-N-MC cells.