상세검색
최근 검색어 전체 삭제
다국어입력
즐겨찾기0
커버이미지 없음
KCI등재 학술저널

The Inhibitory Effects of Hydrogen Sulfide on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine

The Inhibitory Effects of Hydrogen Sulfide on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine

  • 3

In this study, we studied whether hydrogen sulfide (H<sub>2</sub>S) has an effect on the pacemaker activity of interstitial cells of Cajal (ICC), in the small intestine of mice. The actions of H<sub>2</sub>S on pacemaker activity were investigated using whole-cell patch-clamp technique, intracellular Ca<sup>2+</sup> analysis at 30<sup>o</sup>C and RT-PCR in cultured mouse intestinal ICC. Exogenously applied sodium hydrogen sulfide (NaHS), a donor of hydrogen sulfide, caused a slight tonic inward current on pacemaker activity in ICC at low concentrations (50 and 100&lt;/SUP&gt;&#1356;M), but at high concentration (500&#1356;M and 1 mM) it seemed to cause light tonic inward currents and then inhibited pacemaker amplitude and pacemaker frequency, and also an increase in the resting currents in the outward direction. Glibenclamide or other potassium channel blockers (TEA, BaCl<sub>2</sub>, apamin or 4-aminopydirine) did not have an effect on NaHS-induced action in ICC. The exogenous application of carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP) and thapsigargin also inhibited the pacemaker activity of ICC as NaHS. Also, we found NaHS inhibited the spontaneous intracellular Ca<sup>2+</sup> ([Ca<sup>2+</sup>]<sub>i</sub>) oscillations in cultured ICC. In doing an RT-PCR experiment, we found that ICC enriched population lacked mRNA for both CSE and CBS, but was prominently detected in unsorted muscle. In conclusion, H<sub>2</sub>S inhibited the pacemaker activity of ICC by modulating intracellular Ca<sup>2+</sup>. These results can serve as evidence of the physiological action of H<sub>2</sub>S as acting on the ICC in gastrointestinal (GI) motility.

로딩중