Morinda citrifolia Inhibits Both Cytosolic Ca<SUP>2＋</SUP>-dependent Phospholipase A<SUB>2</SUB> and Secretory Ca<SUP>2＋</SUP>-dependent Phospholipase A<SUB>2</SUB>
Morinda citrifolia Inhibits Both Cytosolic Ca<SUP>2＋</SUP>-dependent Phospholipase A<SUB>2</SUB> and Secretory Ca<SUP>2＋</SUP>-dependent Phospholipase A<SUB>2</SUB>
- Ho Sun Song Sung Hun Park Myoung Soo Ko Jae Min Jeong Uy Dong Sohn Sang Soo Sim
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제14권 제3호
- 등재여부 : KCI등재
- 2010.01
- 163 - 167 (5 pages)
This study investigated the effects of the methanol extracts of Morinda citrifolia containing numerous anthraquinone and iridoid on phospholipase A<sub>2</sub> (PLA<sub>2</sub>) isozyme. PLA<sub>2</sub> activity was measured using various PLA<sub>2</sub> substrates, including 10-pyrene phosphatidylcholine, 1-palmitoyl-2-[<sup>14</sup>C]arachidonyl phosphatidylcholine ([<sup>14</sup>C]AA-PC), and [<sup>3</sup>H]arachidonic acid (AA). The methanol extracts suppressed melittin-induced [<sub>3</sub>H]AA release in a concentration-dependent manner in RAW 264.7 cells, and inhibited cPLA<sub>2</sub>/sPLA<sub>2</sub>-induced hydrolysis of [<sup>14</sup>C]AA-PC in a concentration- and time-dependent manner. A Dixon plot showed that the inhibition by methanol extracts on cPLA<sub>2</sub> and sPLA<sub>2</sub> appeared to be competitive with inhibition constants (K<sub>i</sub>) of 3.7Ռg/ml and 12.6Ռg/ml, respectively. These data suggest that methanol extracts of Morinda citrifolia inhibits both Ca<sup>2＋</sup>-dependent PLA<sub>2</sub> such as, cPLA<sub>2</sub> and sPLA<sub>2</sub>. Therefore, Morinda citrifolia may possess anti-inflammatory activity secondary to Ca<sup>2＋</sup>- dependent PLA<sub>2</sub> inhibition.