Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis
Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis
- Changyong Choe Jeong-Won Park Eun-Suk Kim Sung-Gyu Lee Sun-Young Park Jeong-Soon Lee Myung-Je Cho Ke
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제14권 제4호
- 등재여부 : KCI등재
- 2010.01
- 205 - 212 (8 pages)
Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, Ձ-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin Ղ subunit, and potassium channel tetramerisation domain- containing 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, Ձ-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and Ձ-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and Ձ-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.