Anti-inflammatory Activity of 1-docosanoyl Cafferate Isolated from <I>Rhus verniciflua</I> in LPS-stimulated BV2 Microglial Cells
Anti-inflammatory Activity of 1-docosanoyl Cafferate Isolated from <I>Rhus verniciflua</I> in LPS-stimulated BV2 Microglial Cells
- Jae-Won Lee Il-Young Cheong Hae-Sung Kim Jae Jun Lee Yong-Suk Lee Yong-Soo Kwon Myong-Jo Kim Hee Jae
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제15권 제1호
- 등재여부 : KCI등재
- 2011.01
- 9 - 16 (8 pages)
Although various derivatives of caffeic acid have been reported to possess a wide variety of biological activities such as protection of neuronal cells against excitotoxicity, the biological activity of 1-docosanoyl cafferate (DC) has not been examined. The objective of the present study was to evaluate the anti-inflammatory effects of DC, isolated from the stem bark of <i>Rhus verniciflua</i>, on lipopoly</I>saccharide (LPS)-stimulated BV2 microglial cells. Pretreatment of cells with DC significantly attenuated LPS-induced NO production, and mRNA and protein expression of iNOS in a concentration- dependent manner. DC also significantly suppressed LPS-induced release of cytokines such as TNF-Ձ and IL-1Ղ. Consistent with the decrease in cytokine release, DC dose-dependently and significantly attenuated LPS-induced mRNA expression of these cytokines. Furthermore, DC significantly suppressed LPS-induced degradation of IKB, which retains NF-kB in the cytoplasm. Therefore, nuclear translocation of NF-kB induced by LPS stimulation was significantly suppressed with DC pretreatment. Taken together, the present study suggests that DC exerts its anti-inflammatory activity through the suppression of NF-kB translocation to the nucleus.