Genipin Selectively Inhibits TNF-Ձ-activated VCAM-1 But Not ICAM-1 Expression by Upregulation of PPAR-Ճ in Human Endothelial Cells
Genipin Selectively Inhibits TNF-Ձ-activated VCAM-1 But Not ICAM-1 Expression by Upregulation of PPAR-Ճ in Human Endothelial Cells
- Jung Seok Hwa Lidiya Mun Hye Jung Kim Han Geuk Seo Jae Heun Lee Jong Hwan Kwak Dong-Ung Lee Ki Churl
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제15권 제3호
- 등재여부 : KCI등재
- 2011.01
- 157 - 162 (6 pages)
Vascular inflammation process has been suggested to be an important risk factor in the development of atherosclerosis. Recently we reported that induction of peroxisome proliferator-activated receptor-Ճ (PPAR-Ճ) selectively inhibits vascular cell adhesion molecule-1 (VCAM-1) but not intercellular cell adhesion molecule-1 (ICAM-1) in tumor necrosis factor (TNF)-Ձ-activated human umbilical vein endothelial cells (HUVEC). In this study, we investigated whether genipin inhibits expression of cellular adhesion molecules, which is relevant to inflammation. Pretreatment with genipin reduced reactive oxygen species (ROS) production and expression of VCAM-1, but not ICAM-1 in TNF-Ձ-activated HUVEC. Genipin dose- and time-dependently increased PPAR-Ճ expression and inhibited TNF-Ձ-induced phosphorylation of Akt and PKC with different degrees. Finally, genipin prevented TNF-Ձ-induced adhesion of U937 monocytic cells to HUVEC. Taken together, these results indicate that upregualtion of PPAR-Ճ by genipin selectively inhibits TNF-Ձ-induced expression of VCAM-1, in which regulation of Akt and/or PKC play a key role. We concluded that genipin can be used for the treatment of cardiovascular disorders such as atherosclerosis.