The Protective Effect of Quercetin-3-O-Ղ-D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells
The Protective Effect of Quercetin-3-O-Ղ-D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells
- Jung Hyun Cho Sun Young Park Ho Sung Lee Wan Kyunn Whang Uy Dong Sohn
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제15권 제6호
- 등재여부 : KCI등재
- 2011.01
- 319 - 326 (8 pages)
Quercetin-3-O-Ղ-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. We aimed to explore its protective effect against ethanol-induced cell damage and the mechanism involved in the effect in feline esophageal epithelial cells (EEC). Cell viability was tested and 2 ,7 -dichlorofluorescin diacetate assay was used to detect intracellular H<sub>2</sub>O<sub>2</sub> production. Western blotting analysis was performed to investigate MAPK activation and interleukin 6 (IL-6) expression. Exposure of cells to 10% ethanol time-dependently decreased cell viability. Notably, exposure to ethanol for 30 min decreased cell viability to 43.4%. When cells were incubated with 50ՌM QGC for 12 h prior to and during ethanol treatment, cell viability was increased to 65%. QGC also inhibited the H<sub>2</sub>O<sub>2</sub> production and activation of ERK 1/2 induced by ethanol. Pretreatment of cells with the NADPH oxidase inhibitor, diphenylene iodonium, also inhibited the ethanol-induced ERK 1/2 activation. Treatment of cells with ethanol for 30 or 60 min in the absence or presence of QGC exhibited no changes in the IL-6 expression or release compared to control. Taken together, the data indicate that the cytoprotective effect of QGC against ethanol-induced cell damage may involve inhibition of ROS generation and downstream activation of the ERK 1/2 in feline EEC.